p akt ser473 Search Results


phospho akt ser473 antibody  (Proteintech)
96
Proteintech phospho akt ser473 antibody
Phospho Akt Ser473 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/pmc13015967-78-10-15?v=Proteintech
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phospho akt ser473 antibodies  (Novus Biologicals)
92
Novus Biologicals phospho akt ser473 antibodies
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
Phospho Akt Ser473 Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/10__1158_slash_1078___0432__ccr___06___2394-287-5-12?v=Novus+Biologicals
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phospho akt ser473 antibodies - by Bioz Stars, 2026-08
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anti p akt s473 antibodies  (R&D Systems)
92
R&D Systems anti p akt s473 antibodies
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
Anti P Akt S473 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/pm24751383-25-20-25?v=R%26D+Systems
Average 92 stars, based on 1 article reviews
anti p akt s473 antibodies - by Bioz Stars, 2026-08
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anti p akt  (Novus Biologicals)
93
Novus Biologicals anti p akt
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
Anti P Akt, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/10__1158_slash_1078___0432__ccr___13___2284-97-14-16?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
anti p akt - by Bioz Stars, 2026-08
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rabbit anti phospho akt ser 473 antibody  (R&D Systems)
90
R&D Systems rabbit anti phospho akt ser 473 antibody
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
Rabbit Anti Phospho Akt Ser 473 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/pmc03823872-139-0-8?v=R%26D+Systems
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rabbit anti phospho akt ser 473 antibody - by Bioz Stars, 2026-08
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phospho-akt-s473  (ABclonal Biotechnology)
90
ABclonal Biotechnology phospho-akt-s473
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
Phospho Akt S473, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/pmc10730858-233-55-58?v=ABclonal+Biotechnology
Average 90 stars, based on 1 article reviews
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p-akt (ser 473) antibodies  (Becton Dickinson)
90
Becton Dickinson p-akt (ser 473) antibodies
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
P Akt (Ser 473) Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/pmc06539620-105-3-10?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
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phosphorylated p‑akt (ser473) (cat. no. e1a0016)  (EnoGene Inc)
90
EnoGene Inc phosphorylated p‑akt (ser473) (cat. no. e1a0016)
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
Phosphorylated P‑Akt (Ser473) (Cat. No. E1a0016), supplied by EnoGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/pm32952639-34-21-31?v=EnoGene+Inc
Average 90 stars, based on 1 article reviews
phosphorylated p‑akt (ser473) (cat. no. e1a0016) - by Bioz Stars, 2026-08
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p-akt (ser 473) antibody  (G Biosciences)
90
G Biosciences p-akt (ser 473) antibody
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
P Akt (Ser 473) Antibody, supplied by G Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/pm36614133-398-6-12?v=G+Biosciences
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p-akt (ser 473) antibody - by Bioz Stars, 2026-08
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p-akt (ser 473) 28731-1-ap  (PeproTech)
90
PeproTech p-akt (ser 473) 28731-1-ap
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
P Akt (Ser 473) 28731 1 Ap, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p-akt (ser473) (a7004)  (Assay Biotechnology)
90
Assay Biotechnology p-akt (ser473) (a7004)
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
P Akt (Ser473) (A7004), supplied by Assay Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/pm36278136-28-9-15?v=Assay+Biotechnology
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p-akt (ser473) (a7004) - by Bioz Stars, 2026-08
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polyclonal rabbit anti-human anti-p akt (ser473) antibody (cat no. bs4006)  (Bioworld Antibodies)
90
Bioworld Antibodies polyclonal rabbit anti-human anti-p akt (ser473) antibody (cat no. bs4006)
Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT <t>(Ser473)</t> and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.
Polyclonal Rabbit Anti Human Anti P Akt (Ser473) Antibody (Cat No. Bs4006), supplied by Bioworld Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+ser473/pmc04487129-65-18-43?v=Bioworld+Antibodies
Average 90 stars, based on 1 article reviews
polyclonal rabbit anti-human anti-p akt (ser473) antibody (cat no. bs4006) - by Bioz Stars, 2026-08
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Image Search Results


Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT (Ser473) and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.

Journal: Clinical Cancer Research

Article Title: Morin (3,5,7,2′,4′-Pentahydroxyflavone) Abolishes Nuclear Factor-κB Activation Induced by Various Carcinogens and Inflammatory Stimuli, Leading to Suppression of Nuclear Factor-κB–Regulated Gene Expression and Up-regulation of Apoptosis

doi: 10.1158/1078-0432.ccr-06-2394

Figure Lengend Snippet: Fig. 3. A, effect of morin on theTNF-induced activation of IKK. KBM-5 cells (4 106/mL) were pretreated with 50 Amol/L morin for 4 h and then activated with100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and extracts were immunoprecipitated with antibodies against IKK-a and IKK-h.The immune complex kinase assay was then done as described in Materials and Methods.Thereafter, the immunocomplex kinase assay was done as described in Materials and Methods.To examine the effect of morin on the level of expression of IKKa, 50 Ag of cell extract protein were analyzed in 10% SDS-PAGE and assayed for IKKa byWestern blot analysis. B, to detect the direct effect of morin on theTNF-induced activation of IKK, cells were stimulated with100 pmol/LTNF for1h, and then whole-cell extracts were prepared.The extracts were then incubated with different concentrations of morin and immunoprecipitated with anti-IKKa and IKKh antibodies.The immunocomplex kinase assay was then done. C, effect of morin onTNF-induced AKTactivation. Cells (2 106/mL) were incubated with either medium or 50 Amol/L morin for 4 h and then treated with 100 pmol/L TNF for the indicated times.Whole-cell extracts were prepared, and phospho-AKT (Ser473) and AKTwere detected byWestern blot using phospho-specific anti-AKTand anti-AKTantibodies. Results are representative of three independent experiments.

Article Snippet: Anti– IKK-a, anti– IKK-h, and phospho-AKT (Ser473) antibodies were kindly provided by Imgenex (San Diego, CA).

Techniques: Activation Assay, Immunoprecipitation, Immune Complex Kinase Assay, Kinase Assay, Expressing, SDS Page, Incubation